The direct epifluorescent filter technique (DEFT): increased selectivity, sensitivity and rapidity. Rodrigues UM, Kroll RG. With the direct epifluorescent filter technique (DEFT), differentiation of bacteria was achieved by a modified Gram-staining procedure using acridine orange as the counterstain.
Holah, J. T., Betts, R. P., and Thorpe, R. H. (1988) The use of direct epifluorescent microscopy (DEM) and the direct epifluorescent filter technique (DEFT) to assess microbial populations on food contact surfaces. J. Appl. Bacteriol. 65, 215–222. PubMed Google Scholar
R.G. Kroll, U.M. Rodrigues, The direct epifluorescent filter technique, cytochrome c oxidase test and plate count method for predicting the keeping quality of pasteurized cream, Food Microbiology, 10.1016/S0740-0020(86)80041-7, 3, 2, (185-194), (1986).
With the direct epifluorescent filter technique (DEFT), differentiation of bacteria was achieved by a modified Gram‐staining procedure using acridine orange as the counterstain. The method enumerat...
Improved Direct Epifluorescent Filter Technique for Rapid Bioburden Control in Intravenous Solutions. Jose Maria Esteban, Mariano Leiva Diaz, Fabian Soriano and Enrique Marin. PDA Journal of Pharmaceutical Science and Technology September 1992, 46 (5) 146-149; Article
Review: The direct epifluorescent filter technique Review: The direct epifluorescent filter technique PETTIPHER, G. L. 1986-04-01 00:00:00 Summary The direct epifluorescent filter technique (DEFT) for the rapid enumeration of microorganisms is described with particular reference to milk and foods. Recent development with regard to applications and automation, and the likely future of the
Apr 01, 1986· Food Microbiology, 1986, 3, 185-194 The direct epifluorescent filter technique, cytochrome c oxidase test and plate count method for predicting the keeping quality of pasteurized cream R. G. Kroll* and U. M. Rodrigues Department of Food Microbiology, AFRC Institute of Food Research, Reading Laboratory, Shinfield, Reading RG2 9AT, UK Received 20 February 1986 The ability of the direct
Use of the Direct Epifluorescent Filter Technique for predicting the keeping quality of pasteurized milk within 24 hours. Rodrigues UM, Pettipher GL. The keeping quality (KQ) of pasteurized milk stored at 5 degrees C and 11 degrees C was predicted within 24 h by pre-incubating samples and counting bacteria by the Direct Epifluorescent Filter
The light path in epifluorescence microscopes. The illustration below shows the typical light path of an epifluorescence microscope. Most microscopes that are used for cell biology are arranged so that the light travels through the objective lens to illuminate the sample, and then the light emitted from the sample travels back through the same objective to the detector.
The ability of the direct epifluorescent filter technique (DEFT) to enumerate the viable numbers of various species of yeasts was evaluated. A DEFT count could be made in less than 10 min and the
Jul 13, 2019· This technique has also been extensively used in the food industry. It utilizes the observation that viable cells, when exposed to acridine orange, can be visualized under a fluorescent
The direct epifluorescent filter technique (DEFT), an ex-tremelyrapidmicrobiological analyticalmethod,hasbeenused for manyyears for direct quantitation of microbial load in a variety of
This is the first monograph in the Innovation in Microbiology Research Studies Series. It is concerned with all aspects of the direct epifluorescent filter technique (DEFT) for counting bacteria in raw milk. Microorganisms are recovered from the sample by membrane filtration (after treatment of the sample with trypsin and surfactant to disperse fat and somatic cells); cells on the filter
The direct epifluorescent filter technique (DEFT) for the rapid enumeration of microorganisms is described with particular reference to milk and foods. Recent development with regard to applications
Improved Direct Epifluorescent Filter Technique for Rapid Bioburden Control in Intravenous Solutions. Jose Maria Esteban, Mariano Leiva Diaz, Fabian Soriano and Enrique Marin. PDA Journal of
J. T. Holah, R. P. Betts, and R. H. Thorpe (1988) The use of direct epifluorescent microscopy (DEM) and the direct epifluorescent filter technique (DEFT) to asses microbial population on food contact
Direct epifluorescent filter technique (DEFT). Use in assesment of microbiological quality of water. Foreign Title : Direkte epifluorescens filter teknik (DEFT-metoden). Anvendelse til vurdering af
In a modification of the direct epifluorescent filter technique (DEFT), direct fluorescent antibody staining was used for the rapid (<1 h), specific enumeration of foodbome Escherichia coli 0157:H7 by
The light path in epifluorescence microscopes. The illustration below shows the typical light path of an epifluorescence microscope. Most microscopes that are used for cell biology are arranged so that the
The ability of the direct epifluorescent filter technique (DEFT) to enumerate the viable numbers of various species of yeasts was evaluated. A DEFT count could be made in less than 10 min and the DEFT counts of non‐heat‐treated samples agreed well with plate counts.
Jul 13, 2019· This technique has also been extensively used in the food industry. It utilizes the observation that viable cells, when exposed to acridine orange, can be visualized under a fluorescent microscope, appearing bright orange, while nonviable cells appear green.By sample filtration, the viable cells can be stained in situ and immediately counted, giving a result within one hour or less.
Improved Direct Epifluorescent Filter Technique for Rapid Bioburden Control in Intravenous Solutions. Jose Maria Esteban, Mariano Leiva Diaz, Fabian Soriano and Enrique Marin. PDA Journal of Pharmaceutical Science and Technology September 1992, 46 (5) 146-149; Article
The direct epifluorescent filter technique (DEFT) for the rapid enumeration of microorganisms is described with particular reference to milk and foods. Recent development with regard to applications and automation, and the likely future of the method are discussed.
Antibody-direct epifluorescent filter technique for rapid, direct enumeration of Escherichia coli O157:H7 in Beef Article (PDF Available) in Applied and Environmental Microbiology 60(10):3553-9
J. T. Holah, R. P. Betts, and R. H. Thorpe (1988) The use of direct epifluorescent microscopy (DEM) and the direct epifluorescent filter technique (DEFT) to asses microbial population on food contact surfaces. J. Appl. Bacteriol. 65, 215–221. Google Scholar
This is the first monograph in the Innovation in Microbiology Research Studies Series. It is concerned with all aspects of the direct epifluorescent filter technique (DEFT) for counting bacteria in raw milk. Microorganisms are recovered from the sample by membrane filtration (after treatment of the sample with trypsin and surfactant to disperse fat and somatic cells); cells on the filter are...
Oct 01, 1994· Rapid membrane filtration-epifluorescent microscopy technique for direct enumeration of bacteria in raw milk. Appl Environ Microbiol. 1980 Feb; 39 (2):423–429. [Europe PMC free article] [Google Scholar] Pettipher GL, Rodrigues UM. Rapid enumeration of microorganisms in foods by the direct epifluorescent filter technique. Appl Environ Microbiol.
Called the Direct Epifluorescent Filter Technique (DEFT), this method uses dyes and fluorescent microscopy to rapidly enumerate bacteria on a filter. 2. This procedure includes a pre-filtering step to remove large food particles (-if necessary-5µm filter used) and then the filtrate is treated with a detergent and a protease to degrade somatic
The light path in epifluorescence microscopes. The illustration below shows the typical light path of an epifluorescence microscope. Most microscopes that are used for cell biology are arranged so that the light travels through the objective lens to illuminate the sample, and then the light emitted from the sample travels back through the same objective to the detector.
Sigma-Aldrich offers abstracts and full-text articles by [U M Rodrigues, R G Kroll].
Use of the Direct Epifluorescent Filter Technique for the Enumeration of Viable and Total Acetic Acid Bacteria from Vinegar Fermentation May 2003 Journal of Fluorescence 13(3):261-265
With the direct epifluorescent filter technique (DEFT), differentiation of bacteria was achieved by a modified Gram-staining procedure using acridine orange as the counterstain. The method enumerated viable Gram-negative and all Gram-positive bacteria. Counts of clumps of orange fluorescent cells.
Direct epifluorescent filter technique for the rapid enumeration of micro-organisms. Letchworth, Hertfordshire, England : Research Studies Press ; New York : Wiley, 1983 (OCoLC)644149715
Jul 13, 2019· This technique has also been extensively used in the food industry. It utilizes the observation that viable cells, when exposed to acridine orange, can be visualized under a fluorescent microscope, appearing bright orange, while nonviable cells appear green.By sample filtration, the viable cells can be stained in situ and immediately counted, giving a result within one hour or less.
Direct epifluorescent filter technique (DEFT) using acridine orange is a method known for examining the microbial content within food and water. The use of acridine orange in clinical applications has become widely accepted, mainly focusing on highlighting bacteria in blood cultures. Past and present studies comparing acridine orange staining
Use of the Direct Epifluorescent Filter Technique for the Enumeration of Viable and Total Acetic Acid Bacteria from Vinegar Fermentation May 2003 Journal of Fluorescence 13(3):261-265
The direct epifluorescent filter technique or DEFT is a microscopy technique which has been applied to the enumeration of micro-organisms in a range of foods, although it was originally developed for estimating bacterial counts in raw milk. The technique was developed in response to the need for a rapid method for judging the hygienic quality
Direct epifluorescent filter technique for the rapid enumeration of micro-organisms. Letchworth, Hertfordshire, England : Research Studies Press ; New York : Wiley, 1983 (OCoLC)644149715
Sigma-Aldrich offers abstracts and full-text articles by [U M Rodrigues, R G Kroll].
EN 13783 EN 13783 Foodstuffs Detection of irradiated food using Direct Epifluorescent Filter Technique/Aerobic Plate Count (DEFT/APC) Screening method This European Standard specifies a microbiological screening method for the detection of irradiation treatment of herbs and spices, using the combined direct epifluorescent filter technique (DEFT) and aerobic plate count (APC).
Called the Direct Epifluorescent Filter Technique (DEFT), this method uses dyes and fluorescent microscopy to rapidly enumerate bacteria on a filter. 2. This procedure includes a pre-filtering step to remove large food particles (-if necessary-5µm filter used) and then the filtrate is treated with a detergent and a protease to degrade somatic
The light path in epifluorescence microscopes. The illustration below shows the typical light path of an epifluorescence microscope. Most microscopes that are used for cell biology are arranged so that the light travels through the objective lens to illuminate the sample, and then the light emitted from the sample travels back through the same objective to the detector.
Dec 13, 2017· Detection for fecal coliforms
A rapid method for the direct enumeration of somatic cells in fresh and formalin-preserved raw milk is described. Heat treatment at 80°C for 10 mn fixed somatic cells sufficiently to prevent lysis and subsequent dilution allowed the equivalent of at least 0.5 ml milk to be filtered through a 1.0 μm pore size Nuclepore membrane filter.
Foodstuffs — Detection of irradiated food using Direct Epifluorescent Filter Technique/Aerobic Plate Count (DEFT/APC) — Screening method 1 Scope This European Standard specifies a microbiological screening method for the detection of irradiation treatment of herbs and spices, using the combined direct epifluorescent filter technique (DEFT) and
The Direct Epifluorescent Filter Technique (DEFT) employs fluorescent dyes and fluorescent microscopy and it is a rapid method to enumerate microorganisms in food. In this method, the food sample is first homogenized, diluted and filtered through a 5-μm nylon filter; the filtrate is then collected and treated with 2 ml of Triton X-100 and 0.5
İngilizce Türkçe online sözlük Tureng. Kelime ve terimleri çevir ve farklı aksanlarda sesli dinleme. direct epifluorescent filter technique direkt epifloresan filtre tekniği ne demek.