Key Publications. A Phase Separation Model for Transcriptional Control. Hnisz, D, Shrinivas, K, Young, RA, Chakraborty, AK, Sharp, PA. 2017. Cell 169, 13-23.
Richard A. Young's lab is exploring gene regulation in health and disease. super-enhancers led to our proposal that the high-density assembly of biomolecules at active super-enhancers is due to phase separation of enriched factors at these genetic elements (Hnisz et al., 2017). We recently provided experimental evidence that super-enhancers
Richard A. Young. Rick Young is a pioneer in the systems biology of gene control and expression and has harnessed the emergent understanding of biomolecular condensates and phase separation for new insights in his field. He is a member of the Whitehead Institute and Professor of Biology at the Massachusetts Institute of Technology.
Jul 22, 2020· Whitehead Institute Member Richard Young, Phase separation and Rett syndrome. Proteins that form condensates often contain intrinsically
Phase-separated multi-molecular assemblies provide a general regulatory mechanism to compartmentalize biochemical reactions within cells. We propose that a phase separation model explains established and recently described features of transcriptional control. These features include the
Mar 23, 2017· Our phase separation model suggests that this is because stimulation by TNFα led to modifications that change the valency of interacting components, and for SEs, phase separation occurs sharply above a lower value of valency compared to typical enhancers, thus resulting in enhanced recruitment of interacting components such as BRD4 (Figure 2C).
The phase separation model shows that SEs recapitulate the high frequency with low variation (around a relatively constant high transcriptional activity) bursting pattern exhibited by strong enhancers while typical enhancers exhibit more variable bursts with a lower frequency (Figure 4B). Once sustained phase separation occurs (TA saturates
Richard Allen Young (born March 12, 1954) is an American geneticist, a Member of Whitehead Institute, and a professor of biology at the Massachusetts Institute of Technology. He is a pioneer in the systems biology of gene control who has developed genomics technologies and concepts key to understanding gene control in human health and disease. He has served as an advisor to the World Health
There is increasing interest in the function of phase-separated biomolecular condensates in cells because of their distinct properties and expanding roles in important biological processes. Klein et al. considered the fate of small-molecule therapeutics in the context of nuclear condensates (see the Perspective by Viny and Levine). They show that certain antineoplastic drugs have
Jul 27, 2018· Phase separation of fluids is a physicochemical process by which molecules separate into a dense phase and a dilute phase. Phase-separated biomolecular condensates, which include the nucleolus, nuclear speckles, stress granules, and others, provide a mechanism to compartmentalize and concentrate biochemical reactions within cells ( 1 3 ).
Richard A. Young. Rick Young is a pioneer in the systems biology of gene control and expression and has harnessed the emergent understanding of biomolecular condensates and phase separation for new insights in his field. He is a member of the Whitehead Institute and Professor of Biology at the Massachusetts Institute of Technology.
Jul 22, 2020· Whitehead Institute Member Richard Young, Phase separation and Rett syndrome. Proteins that form condensates often contain intrinsically
We propose that a phase separation model explains established and recently described features of transcriptional control. These features include the formation of super-enhancers, the sensitivity of super-enhancers to perturbation, the transcriptional bursting patterns of enhancers, and the ability of an enhancer to produce simultaneous activation at multiple genes.
Dec 27, 2018· Compartmentalization of biochemical processes is essential for cell function. Although membrane‐bound organelles are well studied in this context, recent work has shown that phase separation is a key contributor to cellular compartmentalization through the formation of liquid‐like membraneless organelles (MLOs).
Condensates and Phase Separation in Biology. November 3-4, 2020 General Director: Richard Young, Whitehead Institute, MIT Registration form > Registration deadline: October 31, 2020 See also: Upcoming School, Life Science, 2020-2021.
Richard Allen Young (born March 12, 1954) is an American geneticist, a Member of Whitehead Institute, and a professor of biology at the Massachusetts Institute of Technology. He is a pioneer in the systems biology of gene control who has developed genomics technologies and concepts key to understanding gene control in human health and disease. He has served as an advisor to the World Health
Richard A. Young. The nucleus contains diverse phase-separated condensates that compartmentalize and concentrate biomolecules with distinct physicochemical properties. Here, we investigated
Coactivator condensation at super-enhancers links phase separation and gene control Science. 2018 Jul 27;361(6400):eaar3958. doi: 10.1126/science.aar3958. Epub 2018 Jun 21. Authors Benjamin R Sabari 1 Richard A Young 12 3
d Phase-separation capacity of TFs is associated with gene activation d TF condensates incorporate dynamic and structured interactions Authors Ann Boija, Isaac A. Klein, Benjamin R. Sabari,, Tong Ihn Lee, Dylan J. Taatjes, Richard A. Young Correspondence [email protected] In Brief Activation domains from a diverse array of mammalian and
2018年6月21日Richard Young 课题组在Science发表了题为Coactivator condensation at super-enhancers links phase separation and gene control的文章【9】，该文报道了转录共激活因子BRD4和MED1可以在超级增强子处发生相分离形成液滴，将转录机器聚集在超级增强子附近，实现转录过程的区室
Phase separation is a process by which a homogeneous liquid solution (or phase) of macromolecular components separates (or demixes) into two distinct phases, one phase that is enriched for the macromolecules and another phase that is depleted of the same macromolecules (4, 12, 75, 138). The liquid properties of the dense phase, such as rapid
Analyses of reactions above and below the phase separation threshold lead to a quantitative model in which reactions in condensates are accelerated by mass action and by changes in substrate KM, likely due to scaffold-induced molecular organization. Richard Young & Isaac Klein. October 22 @ 1:00 pm 2:00 pm EDT . Thu 22.
生物学には"スーパーエンハンサー"という用語がある。 歴史はそれほど長くはない。 初めに"スーパーエンハンサー"と言い出したのはRichard Youngという大物である。 2013年に彼がのグループがCellにBack-to-backで出した2報の論文が "スーパーエンハンサー"のおこりである。
Jul 22, 2018· Liquid-like droplets aid these processes by biological “phase-separation” or the selective concentration of some molecules into a small intracellular area and the exclusion of others. Excitingly, two new papers from the labs of Richard A. Young
& Richard A. Young Trends in Biochemical Sciences (2020) Dosage Sensing, Threshold Responses, and Epigenetic Memory: A Systems Biology Perspective on Random X‐Chromosome Inactivation
Nov 21, 2018· Postdocs Ann Boija and Isaac Klein from Whitehead Institute Member Richard Young's lab describe how phase separated condensates appear as
Dec 27, 2018· Compartmentalization of biochemical processes is essential for cell function. Although membrane‐bound organelles are well studied in this context, recent work has shown that phase separation is a key contributor to cellular compartmentalization through the formation of liquid‐like membraneless organelles (MLOs).
Richard A. Young's 476 research works with 82,770 citations and 8,210 reads, including: lncRNA DIGIT and BRD3 protein form phase-separated condensates to regulate endoderm differentiation
Condensates and Phase Separation in Biology. November 3-4, 2020 General Director: Richard Young, Whitehead Institute, MIT Registration form > Registration deadline: October 31, 2020 See also: Upcoming School, Life Science, 2020-2021.
Jul 22, 2018· Liquid-like droplets aid these processes by biological “phase-separation” or the selective concentration of some molecules into a small intracellular area and the exclusion of others. Excitingly, two new papers from the labs of Richard A. Young
Richard A. Young's 469 research works with 81,259 citations and 7,435 reads, including: LIN28B regulates transcription and potentiates MYCN-induced neuroblastoma through binding to ZNF143 at
Phase separation is a process by which a homogeneous liquid solution (or phase) of macromolecular components separates (or demixes) into two distinct phases, one phase that is enriched for the macromolecules and another phase that is depleted of the same macromolecules (4, 12, 75, 138). The liquid properties of the dense phase, such as rapid
Jun 01, 2019· The authors discover that, strikingly, while low concentrations of RNA promote phase separation of FUS in vitro, high concentration of RNA act as a buffer and contrasts phase separation. 49 Yuan Lin, David S.W. Protter, Michael K. Rosen, Roy Parker Formation and maturation of phase-separated liquid droplets by RNA-binding proteins
2018年6月21日Richard Young 课题组在Science发表了题为Coactivator condensation at super-enhancers links phase separation and gene control的文章【9】，该文报道了转录共激活因子BRD4和MED1可以在超级增强子处发生相分离形成液滴，将转录机器聚集在超级增强子附近，实现转录过程的区室
Aug 04, 2020· Bacterial cells are small and were long thought to have little to no internal structure. However, advances in microscopy have revealed that bacteria do indeed contain subcellular compartments. But how these compartments form has remained a mystery. Recent progress in larger, more complex eukaryotic cells has identified a novel mechanism for intracellular organization known
Jul 22, 2020· Whitehead Institute Member Richard Young, also a professor of biology at Massachusetts Institute of Technology (MIT), has been exploring the previously unknown role that condensates play in gathering the molecules needed for gene transcription--the process by which DNA is read into RNA. Phase separation and Rett syndrome. Proteins that form
When cell biologists largely abandoned colloidal phase separation, it was left to relative outsiders agricultural scientists and physicists to make further progress in the study of phase separating biomolecules in cells.. Beginning in the early 1970s, Harold M Farrell Jr. at the US Department of Agriculture developed a colloidal phase separation model for milk casein micelles that form
Sep 11, 2020· Invited Speaker, Richard Young Whitehead Institute/MIT. 02:00 pm 02:20 pm. The role of phase separation mediated by NUP98 fusion oncoproteins in leukemia Bappaditya Chandra St. Jude Children's Research Hospital . 02:20 pm 02:40 pm.
& Richard A. Young Trends in Biochemical Sciences (2020) Dosage Sensing, Threshold Responses, and Epigenetic Memory: A Systems Biology Perspective on Random X‐Chromosome Inactivation
Nov 21, 2018· Postdocs Ann Boija and Isaac Klein from Whitehead Institute Member Richard Young's lab describe how phase separated condensates appear as
Richard A. Young's 476 research works with 82,770 citations and 8,210 reads, including: lncRNA DIGIT and BRD3 protein form phase-separated condensates to regulate endoderm differentiation
Coactivator condensation at super-enhancers links phase separation and gene control: Organism: Mus musculus: Experiment type: Richard A Young: E-mail(s) [email protected]: Phone: 617-258-5219: Organization name: Whitehead Institute for Biomedical Research: Lab: Young Lab: Street address: 9 Cambridge Center: City: Cambridge: State
Sep 17, 2020· The phase separation mechanism helps explain how chromatin is organized in the nucleus and implies that changes of this process may be involved in altering chromatin architecture for carcinogenesis. In addition, several new regulatory elements have been uncovered in the past few years, and their function in normal cells and cancer cells is
May 01, 2002· Phase separation and physico-chemical processes at microscopic and macroscopic levels in MWCNT laden polymer blends using a unique droplet based architecture. Physical Chemistry Chemical Physics 2017, 19 (36),24961-24970. DOI: 10.1039/C7CP03621C.
Jun 01, 2019· The authors discover that, strikingly, while low concentrations of RNA promote phase separation of FUS in vitro, high concentration of RNA act as a buffer and contrasts phase separation. 49 Yuan Lin, David S.W. Protter, Michael K. Rosen, Roy Parker Formation and maturation of phase-separated liquid droplets by RNA-binding proteins
近日Richard Young组在Cell杂志上发表了题为 Transcription Factors Activate Genes through the Phase-Separation Capacity of Their Activation Domains 的文章 【2】 ，报道了转录因子OCT4和GCN4的转录激活结构域通过与转录中介体复合物Mediator发生液-液相分离激活基因表达， 这项研究为我们
2018年6月21日Richard Young 课题组在Science发表了题为Coactivator condensation at super-enhancers links phase separation and gene control的文章【9】，该文报道了转录共激活因子BRD4和MED1可以在超级增强子处发生相分离形成液滴，将转录机器聚集在超级增强子附近，实现转录过程的区室
（2018年6月Richard Young教授在Science发表超级增强子结合的相变文章 Coactivator condensation at super-enhancers links phase separation and gene control的，文章发现转录共激活因子BRD4和MED1可以在超级增强子处聚成液滴样的核斑，并且该聚合可被化学处理干扰。
Disruption of BRD4 and MED1 puncta by 1,6-hexanediol is accompanied by a loss of BRD4 and MED1 at SEs and a loss of RNAPII from SE-driven genes. We find that the intrinsically disordered regions (IDRs) of BRD4 and MED1 are sufficient to form phase-separated droplets in vitro and the MED1 IDR promotes phase separation in living cells.
Background: Accumulated evidence suggests a critical role of protein-mediated liquid-liquid phase separation (LLPS) in many key biological processes through organization of membrane-less compartments. However, the pathological role of protein-mediated LLPS remains elusive and it is unknown whether protein-mediated LLPS contributes to carcinogenesis.
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近日Richard Young组在Cell杂志上发表了题为 Transcription Factors Activate Genes through the Phase-Separation Capacity of Their Activation Domains 的文章 【2】 ，报道了转录因子OCT4和GCN4的转录激活结构域通过与转录中介体复合物Mediator发生液-液相分离激活基因表达， 这项研究为我们